Improvement of (S)-selective imine reductase GF3546 for the synthesis of chiral cyclic amines

Yuta Fukawa; Keita Yoshida; Sayaka Degura; Koichi Mitsukura; Toyokazu Yoshida

doi:10.1039/D2CC05116H

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Improvement of (S)-selective imine reductase GF3546 for the synthesis of chiral cyclic amines†

Yuta Fukawa,^a Keita Yoshida,^b Sayaka Degura,^b Koichi Mitsukura

*^c and Toyokazu Yoshida^c

Author affiliations

* Corresponding authors

^a Department of Engineering Science, Graduate School of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan

^b Department of Life Science and Chemistry, Graduate School of Natural Science and Technology, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan

^c Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
E-mail: mitukura@gifu-u.ac.jp

Abstract

An engineered (S)-selective imine reductase from Streptomyces sp. GF3546 (SIR46) showed twice the activity of the wild type and high thermostability at 50 °C. Using a biocatalyst based on SIR46, we investigated the activity toward high-concentration 2-methyl-1-pyrroline up to 500 mM, conversion rates of other imines, and reductive amination activity.

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Article information

DOI: https://doi.org/10.1039/D2CC05116H
Article type: Communication
Submitted: 16 Sep 2022
Accepted: 04 Nov 2022
First published: 07 Nov 2022

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Chem. Commun., 2022,58, 13222-13225

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Improvement of (S)-selective imine reductase GF3546 for the synthesis of chiral cyclic amines

Y. Fukawa, K. Yoshida, S. Degura, K. Mitsukura and T. Yoshida, Chem. Commun., 2022, 58, 13222 DOI: 10.1039/D2CC05116H

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Chemical Communications

Improvement of (S)-selective imine reductase GF3546 for the synthesis of chiral cyclic amines†

Abstract

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Improvement of (S)-selective imine reductase GF3546 for the synthesis of chiral cyclic amines

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