Selection and characterisation of bioreceptors to develop nanoparticle-based lateral-flow immunoassays in the context of the SARS-CoV-2 outbreak

Liming Hu; Enric Calucho; Celia Fuentes-Chust; Claudio Parolo; Andrea Idili; Ruslan Álvarez-Diduk; Lourdes Rivas; Arben Merkoçi

doi:10.1039/D2LC00486K

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Selection and characterisation of bioreceptors to develop nanoparticle-based lateral-flow immunoassays in the context of the SARS-CoV-2 outbreak†

Liming Hu,

‡^a Enric Calucho,‡^a Celia Fuentes-Chust,^a Claudio Parolo,

^ab Andrea Idili,

^a Ruslan Álvarez-Diduk,

^a Lourdes Rivas

^a and Arben Merkoçi

*^ac

Author affiliations

* Corresponding authors

^a Nanobioelectronics & Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, BIST, Campus UAB, 08193, Bellaterra, Barcelona, Spain
E-mail: arben.merkoci@icn2.cat

^b ISGlobal, Barcelona Centre for International Health Research (CRESIB), Hospital Clínic (Department of International Health), Universitat de Barcelona, Barcelona, Spain

^c ICREA, Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain

Abstract

This manuscript aims at raising the attention of the scientific community to the need for better characterised bioreceptors for fast development of point-of-care diagnostic devices able to support mass frequency testing. Particularly, we present the difficulties encountered in finding suitable antibodies for the development of a lateral flow assay for detecting the nucleoprotein of SARS-CoV-2.

This article is part of the themed collection: Miniaturised Sensors & Diagnostics

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Article information

DOI: https://doi.org/10.1039/D2LC00486K
Article type: Communication
Submitted: 27 May 2022
Accepted: 10 Jun 2022
First published: 29 Jul 2022
This article is Open Access

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Lab Chip, 2022,22, 2938-2943

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Selection and characterisation of bioreceptors to develop nanoparticle-based lateral-flow immunoassays in the context of the SARS-CoV-2 outbreak

L. Hu, E. Calucho, C. Fuentes-Chust, C. Parolo, A. Idili, R. Álvarez-Diduk, L. Rivas and A. Merkoçi, Lab Chip, 2022, 22, 2938 DOI: 10.1039/D2LC00486K

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Selection and characterisation of bioreceptors to develop nanoparticle-based lateral-flow immunoassays in the context of the SARS-CoV-2 outbreak†

Abstract

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Selection and characterisation of bioreceptors to develop nanoparticle-based lateral-flow immunoassays in the context of the SARS-CoV-2 outbreak

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