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RSC Advances

A highly selective and sensitive spectrofluorimetric method for the assessment of 3-nitrotyrosine in serum using (Eu(TTA)3Phen) photo probe

Mohmed S. Attia, ORCID logo *a   Ahmed O. Youssef,a   Nour A. Abdel-Sattar,a   Mohammed A. Amin, ORCID logo b   Sarah Alharthi, ORCID logo b   Ekram H. Mohamed, ORCID logo c   Safwat A. Mahmoud*d  and  Mona N. Abou-Omar*e  

* Corresponding authors

a Chemistry Department, Faculty of Science, Ain Shams University, Cairo 11566, Egypt
E-mail: Mohamed_sam@yahoo.com

b Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

c Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, El Sherouk City, Cairo, Egypt

d Physics Department, Faculty of Science, Northern Border University, Arar, Saudi Arabia
E-mail: samahmoud2002@yahoo.com

e Department of Chemistry, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt
E-mail: mona.na66@yahoo.com

Abstract

A simple, accurate and fast method was developed for the assessment of 3-nitrotyrosine as a biomarker for the early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE) using a (Eu(TTA)3Phen) photo probe. 3-Nitrotyrosine can remarkably quench the luminescence intensity of the (Eu(TTA)3Phen) complex in DMSO at pH = 9 and λem = 617 nm. The quenching of the luminescence intensity of (Eu(TTA)3Phen) complex particularly the electrical emission band at λem = 617 nm is used for the assessment of 3-nitrotyrosine in different serum samples of patients with liver cirrhosis.

Graphical abstract: A highly selective and sensitive spectrofluorimetric method for the assessment of 3-nitrotyrosine in serum using (Eu(TTA)3Phen) photo probe

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Article information

DOI
https://doi.org/10.1039/D1RA07351F
Article type
Paper
Submitted
02 Oct 2021
Accepted
22 Jan 2022
First published
03 Feb 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 4536-4542

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A highly selective and sensitive spectrofluorimetric method for the assessment of 3-nitrotyrosine in serum using (Eu(TTA)3Phen) photo probe

M. S. Attia, A. O. Youssef, N. A. Abdel-Sattar, M. A. Amin, S. Alharthi, E. H. Mohamed, S. A. Mahmoud and M. N. Abou-Omar, RSC Adv., 2022, 12, 4536 DOI: 10.1039/D1RA07351F

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