Issue 17, 2022, Issue in Progress
From the journal:

RSC Advances

Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection†

Rujian Zhao,ab   Chunxu Yu,ab   Baiyang Lu*a  and  Bingling Li ORCID logo *ab  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China
E-mail: bylv@ciac.ac.cn, binglingli@ciac.ac.cn

b School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China

Abstract

We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practical sensitivity as low as ∼aM of ASFV gene in serum.

Graphical abstract: Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection

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Article information

DOI
https://doi.org/10.1039/D2RA01332K
Article type
Paper
Submitted
28 Feb 2022
Accepted
24 Mar 2022
First published
04 Apr 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 10374-10378

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Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection

R. Zhao, C. Yu, B. Lu and B. Li, RSC Adv., 2022, 12, 10374 DOI: 10.1039/D2RA01332K

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