Issue 19, 2022
From the journal:

RSC Advances

Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions†

Tetsuya Hirata, ORCID logo ‡*a   Takahiro Takekiyo,‡*b   Yukihiro Yoshimura,*b   Yuko Tokoro,a   Takeru Ishizaki,c   Yasuhiko Kizukaa  and  Kosuke Kuroda ORCID logo *cd  

* Corresponding authors

a Institute for Glyco-core Research (iGCORE), Gifu University, Gifu, Gifu 501-1193, Japan
E-mail: hirata01@gifu-u.ac.jp

b Department of Applied Chemistry, National Defense Academy, Yokosuka, Kanagawa, Japan
E-mail: take214@nda.ac.jp, muki@nda.ac.jp

c Faculty of Biological Science and Technology, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa, Japan
E-mail: kkuroda@staff.kanazawa-u.ac.jp

d NanoMaterials Research Institute, Kanazawa University, Kakuma-machi, Kanazawa, Japan

Abstract

We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study highlights the potential utility of synthetic zwitterions as novel cryoprotectants.

Graphical abstract: Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

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Article information

DOI
https://doi.org/10.1039/D2RA01575G
Article type
Paper
Submitted
10 Mar 2022
Accepted
07 Apr 2022
First published
14 Apr 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 11628-11631

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Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

T. Hirata, T. Takekiyo, Y. Yoshimura, Y. Tokoro, T. Ishizaki, Y. Kizuka and K. Kuroda, RSC Adv., 2022, 12, 11628 DOI: 10.1039/D2RA01575G

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