Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients

Ayame Mikagi; Ryosuke Tashiro; Tomoo Inoue; Riki Anzawa; Akiho Imura; Takahiro Tanigawa; Tomohisa Ishida; Takashi Inoue; Kuniyasu Niizuma; Teiji Tominaga; Toyonobu Usuki

doi:10.1039/D2RA06009D

Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients†

Ayame Mikagi,

^a Ryosuke Tashiro,

^b Tomoo Inoue,

*^abc Riki Anzawa,^a Akiho Imura,^a Takahiro Tanigawa,^a Tomohisa Ishida,^c Takashi Inoue,^c Kuniyasu Niizuma,

*^de Teiji Tominaga^b and Toyonobu Usuki

*^a

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* Corresponding authors

^a Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, Japan
E-mail: t-usuki@sophia.ac.jp

^b Department of Neurosurgery, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan
E-mail: tomoo49@gmail.com

^c Department of Neurosurgery, Sendai Medical Center, 2-11-12 Miyagino, Miyagino-ku, Sendai 983-8520, Japan

^d Department of Neurosurgical Engineering and Translational Neuroscience, Graduate School of Biomedical Engineering, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan
E-mail: niizuma@nsg.med.tohoku.ac.jp

^e Department of Neurosurgical Engineering and Translational Neuroscience, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan

Abstract

Utilizing chemically synthesized an isotopically labeled internal standard, isodesmosine-¹³C₃,¹⁵N₁, an isotope-dilution LC-MS/MS method was established. Concentrations of desmosine and isodesmosine in plasma of acute cerebral stroke patients and healthy controls were determined. The concentration of desmosines was markedly higher in plasma from acute stroke patients compared with healthy controls. Desmosines are thus novel biomarkers for evaluating the extent of vascular injury after acute cerebral stroke.

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Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients†

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Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients

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