ROS generation strategy based on biomimetic nanosheets by self-assembly of nanozymes

Abstract

Reactive oxygen species (ROS) play an important role in physiology and have been applied in tumor therapy. However, insufficient endogenous H₂O₂ and hypoxia in cancer cells can lead to limited ROS production and poor therapeutic efficacy. Herein, we develop a biomimetic nanosheet material based on the self-assembly of nanozymes that could supply H₂O₂ under acidic conditions and catalyze a cascade of intracellular biochemical reactions to produce ROS under both normoxic and hypoxic conditions without any external stimuli. In this system, the copper peroxide nanosheets (CPNS), which are pH-responsive, were prepared through coordination of H₂O₂ to Cu²⁺ and then modified using ultrafine Pt NPs to form CPNS@Pt. The CPNS could decompose under acidic conditions, allowing the simultaneous release of Fenton catalytic Cu²⁺ and H₂O₂ accompanied by a Fenton-type reaction between them. On the other hand, Pt NPs were also released. The released Pt NPs behave as an oxidase mimic and catalase mimic. In this way, the well-defined CPNS@Pt can not only relieve hypoxic conditions but also generate ROS to induce cell apoptosis, thereby paving the way for the development of a nanozyme with multienzyme activity as a therapeutic strategy.