Spectroscopic/colorimetric dual-mode rapid and ultrasensitive detection of reactive oxygen species based on shape-dependent silver nanostructures

Abstract

Excessive production of reactive oxygen species (ROS) from endogenous and exogenous pathways is linked to oxidative stress and various diseases. Although a variety of ROS probes have been developed, their multistep synthesis strategies and complicated instrumental operating procedures limit their frequent use. In this work, different shaped silver nanostructures including nanoparticles, nanoprisms, and nanocubes were utilized to demonstrate simple spectroscopic and colorimetric techniques for sensitive ROS detection. The nanostructures displayed different sensing behaviours recorded via plasmon tuning with morphological changes upon exposure to ROS. Among the nanostructures, silver nanocubes were found to be extremely efficient in recognising a particular ROS, namely hypochlorite ions. The detection limits of this ROS were calculated to be 23.76 nM, 85.71 nM, and 36.37 nM for silver nanoparticles, nanoprisms, and nanocubes, respectively. A time-dependent microscopic examination was carried out and revealed that the presence of hypochlorite ions deteriorates structural morphologies. The formation of highly reactive chlorite, chlorate, and chloride ions in hypochlorite ion solution was ascribed to the significant spectroscopic and microscopic changes in all the nanostructures. The attenuation of plasmonic peaks and etching of nanostructures by ROS were supported by the increment of the oxidation state of silver. In addition, silver nanocubes were successfully applied to recognize ROS in Spinacia oleracea and real water samples. The results confirm the potentiality of silver nanostructures for sensitive detection of ROS in biological and environmental systems.