Issue 1, 2023
From the journal:

Chemical Communications

Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR†

Omar Eladl, ORCID logo ‡abc   Yudai Yamaoki, ORCID logo ‡ab   Keiko Kondo, ORCID logo a   Takashi Nagata ORCID logo *ab  and  Masato Katahira ORCID logo *ab  

* Corresponding authors

a Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan
E-mail: katahira.masato.6u@kyoto-u.ac.jp, nagata.takashi.6w@kyoto-u.ac.jp

b Graduate School of Energy Science, Kyoto University, Yoshida-hommachi, Sakyo-ku, Kyoto 606-8501, Japan

c Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt

Abstract

We introduced an isotopically labeled RNA aptamer for HIV-1 Tat prepared by E. coli transcription into HeLa cells. We successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study the interaction of the RNA aptamer with argininamide in living human cells with higher resolution.

Graphical abstract: Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR

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Article information

DOI
https://doi.org/10.1039/D2CC05576G
Article type
Communication
Submitted
13 Oct 2022
Accepted
29 Nov 2022
First published
29 Nov 2022

Chem. Commun., 2023,59, 102-105

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Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR

O. Eladl, Y. Yamaoki, K. Kondo, T. Nagata and M. Katahira, Chem. Commun., 2023, 59, 102 DOI: 10.1039/D2CC05576G

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