Issue 29, 2023
From the journal:

Chemical Communications

Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells†

Cherie CY. Wong,a   Lu-Lu Sun,b   Meng-Jiao Liu,c   Eleanor Stride, ORCID logo d   Jason L. Raymond, ORCID logo a   Hai-Hao Han, ORCID logo *bc   James Kwan ORCID logo *a  and  Adam C. Sedgwick ORCID logo *e  

* Corresponding authors

a Department of Engineering Science, Parks Road, Oxford, UK
E-mail: james.kwan@balliol.ox.ac.uk

b Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai, Shandong, P. R. China
E-mail: hanhaihao@simm.ac.cn

c Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P. R. China

d Institute of Biomedical Engineering, Department of Engineering Sciences, Old Road Campus Research Building, University of Oxford, Headington, Oxford, UK

e Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, UK
E-mail: adam.sedgwick@chem.ox.ac.uk

Abstract

We report the synthesis of hydroxyl-radical (˙OH) responsive fluorescent probes that utilise the 3,5-dihydroxybenzyl (DHB) functionality. 4-Methylumbeliferone-DHB (Umb-DHB) and resorufin-DHB (Res-DHB) in the presence of ˙OH radicals resulted in significant increases in their respective fluorescent emission intensities at 460 nm and 585 nm. The incubation of Res-DHB in HeLa cells followed by therapeutic ultrasound (1 MHz) resulted in a significant increase in fluorescence emission intensity thus permitting the ability to monitor ultrasound-induced ˙OH production in live cells.

Graphical abstract: Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells

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Article information

DOI
https://doi.org/10.1039/D3CC00364G
Article type
Communication
Submitted
26 Jan 2023
Accepted
15 Mar 2023
First published
21 Mar 2023
This article is Open Access
Creative Commons BY license

Chem. Commun., 2023,59, 4328-4331

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Fluorescence-based chemical tools for monitoring ultrasound-induced hydroxyl radical production in aqueous solution and in cells

C. CY. Wong, L. Sun, M. Liu, E. Stride, J. L. Raymond, H. Han, J. Kwan and A. C. Sedgwick, Chem. Commun., 2023, 59, 4328 DOI: 10.1039/D3CC00364G

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