A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

Kai-Cheng Yan; Jordan E. Gardiner; Adam C. Sedgwick; Naing Thet; Rachel A. Heylen; Tony D. James; A. Toby A. Jenkins; Xiao-Peng He

doi:10.1039/D3CC00462G

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species†

Kai-Cheng Yan,

^ac Jordan E. Gardiner,

^a Adam C. Sedgwick,

^b Naing Thet,

^a Rachel A. Heylen,

^a Tony D. James,

*^ad A. Toby A. Jenkins

*^a and Xiao-Peng He

*^ce

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Bath, Bath, UK
E-mail: t.d.james@bath.ac.uk, a.t.a.jenkins@bath.ac.uk

^b Chemistry Research Laboratory, University of Oxford, Mansfield Road, UK

^c Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China
E-mail: xphe@ecust.edu.cn

^d School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China

^e National Center for Liver Cancer, The International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, the Second Military Medical University, Shanghai 200433, P. R. China

Abstract

A nitroreductase (NTR) responsive fluorescent probe with long wavelength fluorescence emission was used to determine the NTR activity of a selection of bacterial species under a range of different bacterial growth conditions ensuring applicability under multiple complex clinical environments, where sensitivity, reaction time, and the detection accuracy were suitable for planktonic cultures and biofilms.

This article is part of the themed collection: ChemComm Most Popular 2023 Articles

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DOI: https://doi.org/10.1039/D3CC00462G
Article type: Communication
Submitted: 01 Feb 2023
Accepted: 30 May 2023
First published: 15 Jun 2023
This article is Open Access

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Chem. Commun., 2023,59, 8278-8281

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A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

K. Yan, J. E. Gardiner, A. C. Sedgwick, N. Thet, R. A. Heylen, T. D. James, A. T. A. Jenkins and X. He, Chem. Commun., 2023, 59, 8278 DOI: 10.1039/D3CC00462G

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Chemical Communications

A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species†

Abstract

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A TCF-based fluorescent probe to determine nitroreductase (NTR) activity for a broad-spectrum of bacterial species

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