Issue 67, 2023
From the journal:

Chemical Communications

Extraction-free, one-pot CRISPR/Cas12a detection of microRNAs directly from extracellular vesicles†

He Yan,a   Song Han,b   Steven Hughesb  and  Yong Zeng ORCID logo *acd  

* Corresponding authors

a Department of Chemistry, University of Florida, Gainesville, FL 32611, USA
E-mail: zengy@ufl.edu

b Department of Surgery, University of Florida College of Medicine, Gainesville, FL 32610, USA

c J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA

d University of Florida Health Cancer Center, Gainesville, FL 32611, USA

Abstract

Current methods for extracellular vesicle (EV) miRNA analysis mostly require RNA extraction, which results in a multi-step, time-consuming workflow. This study reports an extraction-free method that combines thermolysis treatment of EVs with a one-pot EXTRA-CRISPR assay, enabling the vastly simplified analysis of EV miRNAs with a comparable performance to that of the extraction-based assays.

Graphical abstract: Extraction-free, one-pot CRISPR/Cas12a detection of microRNAs directly from extracellular vesicles

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Article information

DOI
https://doi.org/10.1039/D3CC02982D
Article type
Communication
Submitted
21 Jun 2023
Accepted
25 Jul 2023
First published
25 Jul 2023

Chem. Commun., 2023,59, 10165-10168

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Extraction-free, one-pot CRISPR/Cas12a detection of microRNAs directly from extracellular vesicles

H. Yan, S. Han, S. Hughes and Y. Zeng, Chem. Commun., 2023, 59, 10165 DOI: 10.1039/D3CC02982D

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