Issue 48, 2023

Excited-state dynamics and fluorescence lifetime of cryogenically cooled green fluorescent protein chromophore anions

Abstract

Time-resolved action spectroscopy together with a fs-pump probe scheme is used in an electrostatic ion-storage ring to address lifetimes of specific vibrational levels in electronically excited states. Here we specifically consider the excited-state lifetime of cryogenically cooled green fluorescent protein (GFP) chromophore anions which is systematically measured across the S0–S1 spectral region (450–482 nm). A long lifetime of 5.2 ± 0.3 ns is measured at the S0–S1 band origin. When exciting higher vibrational levels in S1, the lifetime changes dramatically. It decreases by more than two orders of magnitude in a narrow energy region ∼250 cm−1 (31 meV) above the 0–0 transition. This is attributed to the opening of internal conversion over an excited-state energy barrier. The applied experimental technique provides a new way to uncover even small energy barriers, which are crucial for excited-state dynamics.

Graphical abstract: Excited-state dynamics and fluorescence lifetime of cryogenically cooled green fluorescent protein chromophore anions

Supplementary files

Article information

Article type
Paper
Submitted
27 Sep 2023
Accepted
23 Nov 2023
First published
29 Nov 2023

Phys. Chem. Chem. Phys., 2023,25, 32868-32874

Excited-state dynamics and fluorescence lifetime of cryogenically cooled green fluorescent protein chromophore anions

A. P. Rasmusssen, H. B. Pedersen and L. H. Andersen, Phys. Chem. Chem. Phys., 2023, 25, 32868 DOI: 10.1039/D3CP04696F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements