Fluorescent imaging using novel conjugated polymeric nanoparticles-affimer probes in complex in vitro models of colorectal cancer

Precious Jolugbo; Thomas Willott; Wei-Hsiang Lin; Thomas Maisey; Dermott O'Callaghan; Mark A. Green; David G. Jayne; M. Ibrahim Khot

doi:10.1039/D3NR02160B

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Fluorescent imaging using novel conjugated polymeric nanoparticles-affimer probes in complex in vitro models of colorectal cancer†

Precious Jolugbo,

‡^a Thomas Willott,‡^a Wei-Hsiang Lin,^b Thomas Maisey,

^a Dermott O'Callaghan,^c Mark A. Green,

^cd David G. Jayne^a and M. Ibrahim Khot

*^a

Author affiliations

* Corresponding authors

^a Leeds Institute of Medical Research at St James’, School of Medicine, St James University Hospital, University of Leeds, Leeds, UK
E-mail: M.I.Khot@leeds.ac.uk

^b Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, UK

^c Stream Bio Ltd, Alderley Park, Nether Alderley, Cheshire, UK

^d Department of Physics, Faculty of Natural, Mathematical & Engineering Sciences, King's College London, Strand, London, UK

Abstract

We developed a carcinoembryonic antigen (CEA) conjugated polymer nanoparticle (CPN510-CEA-Af) probe to target CEA-expressing CRC cells in vitro. Its efficacy was evaluated in 2D and 3D cultures of LS174T, LoVo, and HT29 CRC cell lines. CPN510-CEA-Af produced greater fluorescent signal intensity than unconjugated particles in both 2D cells and 3D spheriods, indicating its potential as a probe for image-guided colorectal cancer surgery.

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DOI: https://doi.org/10.1039/D3NR02160B
Article type: Communication
Submitted: 09 May 2023
Accepted: 14 Jul 2023
First published: 17 Jul 2023
This article is Open Access

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Nanoscale, 2023,15, 12476-12480

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Fluorescent imaging using novel conjugated polymeric nanoparticles-affimer probes in complex in vitro models of colorectal cancer

P. Jolugbo, T. Willott, W. Lin, T. Maisey, D. O'Callaghan, M. A. Green, D. G. Jayne and M. I. Khot, Nanoscale, 2023, 15, 12476 DOI: 10.1039/D3NR02160B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Fluorescent imaging using novel conjugated polymeric nanoparticles-affimer probes in complex in vitro models of colorectal cancer†

Abstract

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Fluorescent imaging using novel conjugated polymeric nanoparticles-affimer probes in complex in vitro models of colorectal cancer

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