Issue 13, 2023

Identification of peptide epitopes of the gp120 protein of HIV-1 capable of inducing cellular and humoral immunity

Abstract

The Human Immunodeficiency Virus (HIV-1) causes Acquired Immunodeficiency Syndrome (AIDS) and a high percentage of deaths. Therefore, it is necessary to design vaccines against HIV-1 for the prevention of AIDS. Bioinformatic tools and theoretical algorisms allow us to understand the structural proteins of viruses to develop vaccines based on immunogenic peptides (epitopes). In this work, we identified the epitopes: P1, P2, P10, P27 and P30 from the gp120 protein of HIV-1. These peptides were administered intranasally alone or with cholera toxin (CT) to BALB/c mice. The population of CD4+, CD8+ T lymphocytes and B cells (CD19/CD138+, IgA+ and IgG+) from nasal-associated lymphoid tissue, nasal passages, cervical and inguinal nodes was determined by flow cytometry. In addition, anti-peptides IgG and IgA from serum, nasal and vaginal washings were measured by ELISA. The results show that peptides administered by i.n. can modulate the immune response of T and B lymphocyte populations, as well as IgA and IgG antibodies secretion in the different sites analyzed. In conclusion, bioinformatics tools help us to select peptides with physicochemical properties that allow the induction of the humoral and cellular responses that depend on the peptide sequence.

Graphical abstract: Identification of peptide epitopes of the gp120 protein of HIV-1 capable of inducing cellular and humoral immunity

Supplementary files

Article information

Article type
Paper
Submitted
22 Dec 2022
Accepted
12 Mar 2023
First published
20 Mar 2023
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2023,13, 9078-9090

Identification of peptide epitopes of the gp120 protein of HIV-1 capable of inducing cellular and humoral immunity

J. García-Machorro, M. Gutiérrez-Sánchez, D. A. Rojas-Ortega, M. Bello, S. Andrade-Ochoa, S. Díaz-Hernández, J. Correa-Basurto and S. Rojas-Hernández, RSC Adv., 2023, 13, 9078 DOI: 10.1039/D2RA08160A

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