Effects of NO3−, Cl−, and CH3COO− anions and diethylene glycol on the morphological, structural, antidiabetic, and cell viability properties of CeO2 nanoparticles

Abstract

Cerium oxide (CeO₂) nanoparticles (NPs) were synthesized using a modified conventional polyol method. The ratio of diethylene glycol (DEG) and water in the synthesis was varied, and three different cerium precursor salts (Ce(NO₃)₃, CeCl₃, and Ce(CH₃COO)₃) were used. The structure, size, and morphology of the synthesized CeO₂ NPs were studied. An average crystallite size of 13 to 33 nm was obtained from the XRD analysis. Spherical and elongated morphologies of the synthesized CeO₂ NPs were acquired. Average particle sizes in the range of 16–36 nm were obtained by varying different ratios of DEG and water. The presence of DEG molecules on the surface of CeO₂ NPs was confirmed using FTIR. Synthesized CeO₂ NPs were used to study the antidiabetic and cell viability (cell cytotoxicity) properties. Antidiabetic studies were carried out using α-glucosidase enzymes inhibition activity. CeO₂ synthesized using Ce(NO₃)₃ and CeCl₃ precursors showed approximately 40.0% α-glucosidase enzyme inhibition activity, while CeO₂ synthesized using Ce(CH₃COO)₃ showed the lowest α-glucosidase enzyme inhibition activity. Cell viability properties of CeO₂ NPs were investigated using an in vitro cytotoxicity test. CeO₂ NPs prepared using Ce(NO₃)₃ and CeCl₃ were non-toxic at lower concentrations, while CeO₂ NPs prepared using Ce(CH₃COO)₃ were non-toxic at all concentrations. Therefore, polyol-mediated synthesized CeO₂ NPs showed quite good α-glucosidase inhibition activity and biocompatibility.