Volume 2, 2023
From the journal:

Sensors & Diagnostics

Engineering thermostable affinity proteins for use in high-throughput immunoassay formats†

Huan Jia, ORCID logo ‡a   Nazirulmubin Abdul Moomen,‡a   Jeanette Leong,‡a   Patthara Kongsuphol,‡a   Zhi Feng Sherman Lim, ORCID logo a   Carmen Sze Min Pui,a   Yuxuan Tan,a   Ki-Joo Sungb  and  Hadley D. Sikes ORCID logo *ab  

* Corresponding authors

a Singapore-MIT Alliance for Research and Technology Centre, Antimicrobial Resistance, 1 CREATE Way #03-10/11 Innovation Wing, #03-12/13/14 Enterprise Wing, Singapore
E-mail: sikes@mit.edu

b Massachusetts Institute of Technology, Chemical Engineering, Cambridge, Massachusetts, USA

Abstract

ELISA utilizes polystyrene materials for capture reagents. Natural absorption randomly orients the capture reagent, decreasing immobilization and target biomarker accessibility. A thermostable affinity protein rcSso7d can replace antibodies as a capture reagent in cellulose-based diagnostic tests. ‘RAFIASRRIRRP’ at the N-terminus of two repeating units of rcSso7d improved polystyrene immobilization for high-throughput ELISA.

Graphical abstract: Engineering thermostable affinity proteins for use in high-throughput immunoassay formats

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Article information

DOI
https://doi.org/10.1039/D3SD00041A
Article type
Communication
Submitted
14 Feb 2023
Accepted
17 Apr 2023
First published
25 Apr 2023
This article is Open Access
Creative Commons BY-NC license

Sens. Diagn., 2023,2, 627-631

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Engineering thermostable affinity proteins for use in high-throughput immunoassay formats

H. Jia, N. A. Moomen, J. Leong, P. Kongsuphol, Z. F. S. Lim, C. S. M. Pui, Y. Tan, K. Sung and H. D. Sikes, Sens. Diagn., 2023, 2, 627 DOI: 10.1039/D3SD00041A

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