Volume 2, 2023

Salivary endotoxin detection using combined mono/polyclonal antibody-based sandwich-type lateral flow immunoassay device

Abstract

A point-of-care/use lateral flow assay (LFA) is reported for the detection of P. gingivalis endotoxin, a major saliva biomarker for oral health. Two different approaches of sandwich LFA design using either the combination of mono- and polyclonal antibodies or polyclonal antibody only have been evaluated to detect P. gingivalis endotoxins, having a limit of detection of ∼22 ng mL−1 and 46.5 ng mL−1 for water- and saliva-based samples, respectively. The LFA also exhibits good selectivity to P. gingivalis endotoxin versus other endotoxins and proteins. Saliva pretreatment combining syringe filtration and potato starch successfully inhibits α-amylase activity and provides improved results on LFA devices.

Graphical abstract: Salivary endotoxin detection using combined mono/polyclonal antibody-based sandwich-type lateral flow immunoassay device

Supplementary files

Article information

Article type
Paper
Submitted
22 Jun 2023
Accepted
18 Aug 2023
First published
21 Aug 2023
This article is Open Access
Creative Commons BY-NC license

Sens. Diagn., 2023,2, 1460-1468

Salivary endotoxin detection using combined mono/polyclonal antibody-based sandwich-type lateral flow immunoassay device

D. Han, S. Xie and A. J. Steckl, Sens. Diagn., 2023, 2, 1460 DOI: 10.1039/D3SD00158J

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