Issue 8, 2023

Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applications

Abstract

An enzyme-encapsulated silica-based hybrid material was developed using a chitosan gel. Fusion silicatein (InaKC-ChBD-Sil), silicatein fused with a soluble tag and chitin-binding domain, was employed as an interfacial catalyst to form silica on a chitosan gel matrix under physiological conditions, and horseradish peroxidase was immobilised on the hybrid material. Silica formation on the gel was verified via fluorescence microscopy using a designed fusion protein called TBP-mCherry, a fluorescent protein fused with a silica-binding peptide. We report a chitosan gel–silica hybrid material capable of encapsulating enzymes for biomedical and environmental applications.

Graphical abstract: Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applications

Supplementary files

Article information

Article type
Communication
Submitted
27 Nov 2022
Accepted
07 Feb 2023
First published
08 Feb 2023
This article is Open Access
Creative Commons BY-NC license

J. Mater. Chem. B, 2023,11, 1654-1658

Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applications

K. Godigamuwa, K. Nakashima, S. Tsujitani, R. Naota, I. Maulidin and S. Kawasaki, J. Mater. Chem. B, 2023, 11, 1654 DOI: 10.1039/D2TB02581G

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