Designing a novel paper-based microfluidic disc for rapid and simultaneous determination of multiple nutrient salts in water

Abstract

In the face of worsening water quality and escalating water environmental emergencies, this study developed a paper-based microfluidic disk for rapid, on-site determination of ammonia nitrogen, nitrates, nitrites, and phosphates in water. The method utilizes centrifugal microfluidics and paper-based technology, thus simplifying the operation while eliminating the need for on-site reagent preparation. Experimental results demonstrate that the disk requires only 80 microliters of a water sample and 2 minutes to complete the quantitative analysis of the four nutrients, with a coefficient of variation below 1.72% and spike recoveries ranging from 92% to 113%. The development of the disk provides an effective and rapid, on-site testing tool for water quality analysis.