Issue 51, 2024

Use of a head-to-tail peptide cyclase to prepare hybrid RiPPs

Abstract

Cyclotides and lanthipeptides are cyclic peptide natural products with promising bioengineering potential. No peptides have been isolated that contain both structural motifs defining these two families, an N-to-C cyclised backbone and lanthionine linkages. We combined their biosynthetic machineries to produce hybrid structures that possess improved activity or stability, demonstrate how the AEP-1 plant cyclase can be utilised to complete the maturation of the sactipeptide subtilosin A, and present head-to-tail cyclisation of the glycocin sublancin. These studies show the plasticity of AEP-1 and its utilisation alongside other post-translational modifications.

Graphical abstract: Use of a head-to-tail peptide cyclase to prepare hybrid RiPPs

Supplementary files

Article information

Article type
Communication
Submitted
04 Oct 2023
Accepted
25 Apr 2024
First published
04 Jun 2024
This article is Open Access
Creative Commons BY license

Chem. Commun., 2024,60, 6508-6511

Use of a head-to-tail peptide cyclase to prepare hybrid RiPPs

T. Le, D. Zhang, R. M. Martini, S. Biswas and W. A. van der Donk, Chem. Commun., 2024, 60, 6508 DOI: 10.1039/D3CC04919A

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