Delivery of external proteins into the cytoplasm using protein capsules modified with IgG on the surface, created from the amphiphilic two helix-bundle protein OLE-ZIP

Kousuke Takahashi; Taiki Nishiyama; Naoki Umezawa; Yasumichi Inoue; Isamu Akiba; Takehisa Dewa; Atsushi Ikeda; Toshihisa Mizuno

doi:10.1039/D3CC05347D

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Delivery of external proteins into the cytoplasm using protein capsules modified with IgG on the surface, created from the amphiphilic two helix-bundle protein OLE-ZIP†

Kousuke Takahashi,^a Taiki Nishiyama,^a Naoki Umezawa,

^b Yasumichi Inoue,

^b Isamu Akiba,

^c Takehisa Dewa,

^ad Atsushi Ikeda

^e and Toshihisa Mizuno

*^ad

Author affiliations

* Corresponding authors

^a Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi 466-8555, Japan
E-mail: mizuno.toshihisa@nitech.ac.jp

^b Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan

^c Faculty of Environmental Engineering, the University of Kitakyushu, 1-1 Hibikino, Wakamatsu, Kitakyushu, Fukuoka 808-0135, Japan

^d Department of Nanopharmaceutical Sciences, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi 466-8555, Japan

^e Applied Chemistry Program, Graduate School of Advanced Science and Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan

Abstract

This study explores a new method for delivering therapeutic proteins into specific cells using OLE-ZIP capsules that present IgG. OLE-ZIP capsules is a spherical caspules prepared from amphihilic dimetic coiled-coil peptide, OLE-ZIP. Upon presenting cetuximab, these capsules showed preferential uptake in A431 cells and increased cytotoxicity when loaded with RNase A.

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Article information

DOI: https://doi.org/10.1039/D3CC05347D
Article type: Communication
Submitted: 31 Oct 2023
Accepted: 15 Dec 2023
First published: 18 Dec 2023

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Chem. Commun., 2024,60, 968-971

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Delivery of external proteins into the cytoplasm using protein capsules modified with IgG on the surface, created from the amphiphilic two helix-bundle protein OLE-ZIP

K. Takahashi, T. Nishiyama, N. Umezawa, Y. Inoue, I. Akiba, T. Dewa, A. Ikeda and T. Mizuno, Chem. Commun., 2024, 60, 968 DOI: 10.1039/D3CC05347D

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Chemical Communications

Delivery of external proteins into the cytoplasm using protein capsules modified with IgG on the surface, created from the amphiphilic two helix-bundle protein OLE-ZIP†

Abstract

Supplementary files

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Delivery of external proteins into the cytoplasm using protein capsules modified with IgG on the surface, created from the amphiphilic two helix-bundle protein OLE-ZIP

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