Issue 100, 2024
From the journal:

Chemical Communications

ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead†

Masumi Taki, ORCID logo *ab   Masayasu Kuwahara, ORCID logo c   Chaohui Li,a   Naoko Tomoda,d   Naoyuki Miyashita,af   Tetsuo Kana  and  Jay Yang*ade  

* Corresponding authors

a Department of Engineering Science, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC), 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan
E-mail: taki@pc.uec.ac.jp
Tel: +81-42-443-5980

b Institute for Advanced Science, UEC, Chofu 182-8585, Japan

c Graduate School of Integrated Basic Sciences, Nihon University, Setagaya-ku, Tokyo 156-8550, Japan

d Department of GI Surgery II, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, Japan

e School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53706, USA
E-mail: jyang75@wisc.edu

f Department of Biological Systems Engineering, Graduate School of Biology-Oriented Science and Technology, KINDAI University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan

Abstract

A covalent binder for a target protein was obtained by a direct single-round screening of a latent-warhead-modified DNA library via affinity/reactivity-based co-selection of aptameric deoxyribonucleic acid (ARCaDia), followed by a top k-mer analysis. The optimal position of the conjugated warhead on the selected aptamer was simultaneously identified.

Graphical abstract: ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead

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Article information

DOI
https://doi.org/10.1039/D4CC04594G
Article type
Communication
Submitted
06 Sep 2024
Accepted
06 Nov 2024
First published
08 Nov 2024
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2024,60, 14964-14967

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ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead

M. Taki, M. Kuwahara, C. Li, N. Tomoda, N. Miyashita, T. Kan and J. Yang, Chem. Commun., 2024, 60, 14964 DOI: 10.1039/D4CC04594G

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