Issue 4, 2024
From the journal:

Catalysis Science & Technology

Modulating stereoselectivity and catalytic efficiency of carbenoid reactions catalysed by self-sufficient P450s†

Binhao Wang,a   Cuiping You,a   Guochao Xu ORCID logo *a  and  Ye Ni ORCID logo *a  

* Corresponding authors

a Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China
E-mail: guochaoxu@jiangnan.edu.cn, yni@jiangnan.edu.cn

Abstract

Self-sufficient P450s are versatile biocatalysts. Among six self-sufficient P450 backbones, two variants from Tepidiphilus thermophilus exhibited a high TOF value of 1.5 min−1 (P450TT-C385S) and excellent diastereoselectivity of deE = 95.4% (P450TT-C385H). Molecular simulation was performed to reveal the different catalytic mechanisms among the self-sufficient P450 backbones. This work could provide useful information for exploring challenging unnatural reactions.

Graphical abstract: Modulating stereoselectivity and catalytic efficiency of carbenoid reactions catalysed by self-sufficient P450s

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Article information

DOI
https://doi.org/10.1039/D3CY01258A
Article type
Communication
Submitted
10 Sep 2023
Accepted
29 Nov 2023
First published
08 Dec 2023

Catal. Sci. Technol., 2024,14, 835-839

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Modulating stereoselectivity and catalytic efficiency of carbenoid reactions catalysed by self-sufficient P450s

B. Wang, C. You, G. Xu and Y. Ni, Catal. Sci. Technol., 2024, 14, 835 DOI: 10.1039/D3CY01258A

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