Issue 2, 2024

Precise and rapid point-of-care quantification of albumin levels in unspiked blood using organic field-effect transistors

Abstract

Nanowire-based field-effect transistors (FETs) are widely used to detect biomolecules precisely. However, the fabrication of such devices involves complex integration procedures of nanowires into the device and most are not easily scalable. In this work, we report a straightforward fabrication approach that utilizes the grain boundaries of the semiconducting film of organic FETs to fabricate biosensors for the detection of human serum albumin (HSA) with an enhanced sensitivity and detection range. We used trichromophoric pentapeptide (TPyAlaDo-Leu-ArTAA-Leu-TPyAlaDo, TPP) as a receptor molecule to precisely estimate the concentration of HSA protein in human blood. Bi-layer semiconductors (pentacene and TPP) were used to fabricate the OFET, where the pentacene molecule acted as a conducting channel and TPP acted as a receptor molecule. This approach of engineering the diffusion of receptor molecules into the grain boundaries is crucial in developing OFET-based HSA protein sensors, which cover a considerable detection range from 1 pM to 1 mM in a single device. The point-of-care detection in unspiked blood samples was confirmed at 4.2 g dL−1, which is similar to 4.1 g dL−1 measured using a pathological procedure.

Graphical abstract: Precise and rapid point-of-care quantification of albumin levels in unspiked blood using organic field-effect transistors

Supplementary files

Article information

Article type
Paper
Submitted
27 Jul 2023
Accepted
12 Dec 2023
First published
13 Dec 2023
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2024,6, 630-637

Precise and rapid point-of-care quantification of albumin levels in unspiked blood using organic field-effect transistors

A. Mandal, S. Mandal, S. Mallik, S. Mondal, S. S. Bag and D. K. Goswami, Nanoscale Adv., 2024, 6, 630 DOI: 10.1039/D3NA00564J

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