FlkO, a penicillin-binding protein-type thioesterase in cyclofaulknamycin biosynthesis

Abstract

Penicillin-binding protein-type thioesterases (PBP-type TEs) catalyze head-to-tail macrolactamization in bacterial nonribosomal peptide biosynthesis. Here the scope of FlkO, a new PBP-type TE in cyclofaulknamycin biosynthesis, was thoroughly evaluated. The preference for small residues at the substrate C-terminus was consistent with the decreased volume of its putative substrate-binding pocket.