Issue 19, 2024

Two populations of protein molecules detected by small-angle neutron and X-ray scattering (SANS and SAXS) in lyophilized protein:lyoprotector (disaccharide) systems

Abstract

Two protein interaction peaks are observed in pharmaceutically-relevant protein (serum albumin) : disaccharide 1 : 1 and 1 : 3 (w/w) freeze-dried systems for the first time. In samples with a higher disaccharide content, the protein–protein distances are longer for both populations, while the fraction of the protein population with a shorter protein–protein distance is lower. Both factors would favor better stability against aggregation for disaccharide-rich protein formulations. This study provides direct experimental support for a “dilution” hypothesis as a potential stabilization mechanism for freeze-dried protein formulations.

Graphical abstract: Two populations of protein molecules detected by small-angle neutron and X-ray scattering (SANS and SAXS) in lyophilized protein:lyoprotector (disaccharide) systems

Supplementary files

Article information

Article type
Communication
Submitted
08 Jan 2024
Accepted
22 Apr 2024
First published
23 Apr 2024

Soft Matter, 2024,20, 3897-3900

Two populations of protein molecules detected by small-angle neutron and X-ray scattering (SANS and SAXS) in lyophilized protein:lyoprotector (disaccharide) systems

V. Cristiglio, S. Feng, M. Sztucki, X. Yuan and E. Shalaev, Soft Matter, 2024, 20, 3897 DOI: 10.1039/D4SM00028E

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