Issue 9, 2024

A multi-biocatalytic system for effective fumarate synthesis from pyruvate and gaseous CO2

Abstract

Fumarate, an unsaturated dicarboxylic acid, is an important material for producing unsaturated polyester resins and biodegradable plastics. Fumarate synthesis from petroleum-derived benzene and butane as starting materials is expected to be replaced by synthesis methods from renewable raw materials. In this work, fumarate synthesis from gaseous CO2 and pyruvate in an aqueous medium using a multi-biocatalytic system consisting of pyruvate carboxylase (PC), malate dehydrogenase (MDH) and fumarase (FUM) in the presence of ATP and NADH is accomplished. The conversion yield of fumarate from pyruvate using this system was estimated to be approximately 16% after 5 h of incubation.

Graphical abstract: A multi-biocatalytic system for effective fumarate synthesis from pyruvate and gaseous CO2

Supplementary files

Article information

Article type
Communication
Submitted
23 Dec 2023
Accepted
19 Jul 2024
First published
22 Jul 2024
This article is Open Access
Creative Commons BY-NC license

RSC Sustain., 2024,2, 2491-2495

A multi-biocatalytic system for effective fumarate synthesis from pyruvate and gaseous CO2

M. Takeuchi and Y. Amao, RSC Sustain., 2024, 2, 2491 DOI: 10.1039/D3SU00486D

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