Issue 41, 2024

The blue shift of fluorescence emission reveals the dsRNA-loading capacity of cationic nanocarriers

Abstract

The loading capacity of dsRNA by nanocarriers is a key parameter in the process of RNAi commercialisation. In this research, a sustainable, simple, and cheap method was developed to determine the dsRNA loading capacity by popular cationic nanocarriers. In this method, the fluorescence emission of the cationic nanocarrier + (dsRNA–DAPI) shows blue shift in comparison to that of dsRNA–DAPI. When dsRNA–DAPI is completely loaded by cationic nanocarriers, the fluorescence peak coincides with the emission wavelength of DNA–DAPI. The samples of nanocarrier + (dsRNA–DAPI) are simply tested in a fluorometer with no damage to the samples. The reported method is simpler, cheaper, and more sustainable than gel electrophoresis and HPLC, and will fulfill the industry need for reliable and quick quality administration/control in the production process.

Graphical abstract: The blue shift of fluorescence emission reveals the dsRNA-loading capacity of cationic nanocarriers

Supplementary files

Transparent peer review

To support increased transparency, we offer authors the option to publish the peer review history alongside their article.

View this article’s peer review history

Article information

Article type
Communication
Submitted
28 Jun 2024
Accepted
30 Sep 2024
First published
01 Oct 2024

J. Mater. Chem. B, 2024,12, 10559-10562

The blue shift of fluorescence emission reveals the dsRNA-loading capacity of cationic nanocarriers

P. Li, J. Ma, S. Jiang, W. Peng, Y. Huang, R. Zhang and Z. P. Xu, J. Mater. Chem. B, 2024, 12, 10559 DOI: 10.1039/D4TB01393J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements