Lactobacillus-derived artificial extracellular vesicles for skin rejuvenation and prevention of photo-aging†

Abstract

Extracellular vesicles (EVs) are small membrane-bound sacs released by cells that play crucial roles in intercellular communication. They transport biomolecules between cells and have both diagnostic and therapeutic potential. Artificial exosomes, designed to mimic natural exosomes, have been developed using various methods. In this study, Lactobacillus plantarum was used to create Llactobacillus-derived artificial exosomes (LAEs) for skin rejuvenation and anti-aging. LAEs demonstrated monodispersity and effectively improved adverse gene expression and wound healing in fibroblasts. They also modulated aging-related genes and improved skin conditions in humans. Their simplicity, promptness, and lack of animal-derived sources make LAEs a promising alternative to natural exosomes. LAEs have the potential to overcome the technical limitations of artificial exosomes and advance exosome-based technologies for comprehensive skin rejuvenation.