Functional properties of insoluble dietary fibers extracted from different grape pomaces during simulated digestion and in vitro fermentation

Abstract

This study investigated insoluble dietary fibers (IDFs) extracted from the grape pomaces of Cabernet Sauvignon (CS-IDF), Marselan (MS-IDF), and Merlot (ML-IDF). It explored the release patterns and potential bioactivities of dietary fiber-bound polyphenols from these sources through simulated digestion and in vitro colonic fermentation. The results showed a higher polyphenol content in MS grape skins, which also yielded more IDF. Bound polyphenols were released more effectively during fermentation than during digestion. Caffeic acid and epicatechin disappeared during the fermentation stage, while compounds such as chlorogenic acid, catechin, and myricetin appeared. Gentisic acid was the most abundant monomeric phenolic compound in the fermentation fluid. The released polyphenols exhibited strong antioxidant properties and digestive enzyme inhibitory activity. Fermentation of the IDFs increased propionic acid and total short-chain fatty acid (SCFA) levels, particularly in the CS-IDF and MS-IDF groups. MS-IDF also elevated the relative abundance of Acidaminococcus fermentans, a key SCFA producer. Additionally, all IDFs promoted the growth of beneficial gut bacteria such as Bacteroides H uniformis and Phascolarctobacterium A faecium, while reducing harmful bacteria such as Escherichia. Correlation analysis revealed a positive relationship between released polyphenols and the relative abundance of beneficial gut bacteria, including Parabacteroides B 862006 distasonis and Mitsuokella multacida. These findings suggest that dietary fiber-bound polyphenols exhibit significant bioactivity in the gastrointestinal tract, with MS-IDF showing particular advantages in promoting gut health and bioactive compound release.