A naphthalimide-derived chemosensor for ratiometric detection of sulphide ions: insights into the S2−-driven reduction cascade, real-time applications and live cell imaging of bacterial cells

Abstract

Hydrogen sulphide (H2S) is an unpleasant, harmful gas commonly found in the environment, released from geothermal vents, and produced as a byproduct in industries such as oil refining and wastewater treatment. Because of its extreme toxicity, there is growing concern about its presence, necessitating timely detection to ensure human welfare. However, detecting H2S in various environments, including air and water, remains a significant challenge. To develop a probe for sulphide ion detection, herein, we report the synthesis of a highly selective, sensitive, and colorimetric chemosensor (NATRP) for the detection of sulphide ions (S2−) in a 50% aqueous medium. NATRP demonstrates exceptional sensitivity and selectivity for S2− ions relative to other ions, with a limit of quantification of 26 nM and a detection limit of 7.9 nM. It shows aggregation-induced emission quenching, which upon the addition of S2− ions, disaggregates with enhancement in fluorescence intensity. This enables NATRP to detect S2− ions within 15 seconds and it demonstrates good pH stability, suggesting that NATRP can detect sulphide ions across a broad pH range. The mechanism underlying the detection involves the reduction of azide groups to amine groups in the presence of S2− ions, confirmed by NMR titrations and HRMS analysis. Furthermore, NATRP successfully detects S2− ions in water, serum and solid samples, as well as in live cell imaging in bacterial cells. Moreover, UV-visible and fluorescence data have been employed to construct 1-to-2 decoders.

Graphical abstract: A naphthalimide-derived chemosensor for ratiometric detection of sulphide ions: insights into the S2−-driven reduction cascade, real-time applications and live cell imaging of bacterial cells

Supplementary files

Article information

Article type
Paper
Submitted
06 Feb 2025
Accepted
13 May 2025
First published
16 May 2025
This article is Open Access
Creative Commons BY license

Mater. Adv., 2025, Advance Article

A naphthalimide-derived chemosensor for ratiometric detection of sulphide ions: insights into the S2−-driven reduction cascade, real-time applications and live cell imaging of bacterial cells

S. Gupta, G. Kumar, V. Luxami and K. Paul, Mater. Adv., 2025, Advance Article , DOI: 10.1039/D5MA00103J

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