Calcium Alginate Microcapsules Loaded with Vanillin-Chitosan Complex for improving biosafety and bioavailability

Abstract

Despite the promising application, the facile and controllable preparation of zinc-loaded microcapsules is compulsory, especially for biosafety and high bioavailable as well.Here, calcium alginate microcapsules loaded with vanillin-chitosan Schiff base zinc (VCSB-Zn(II)@CA) were successfully primed via emulsification exogenous for Zn2+ continuously sustained release. The microcapsules were characterized by FT-IR, SEM, and TGA. The best preparation condition was obtained by a multi-factor(MF) orthogonal experiment, which was 50 g/L of CaCl2, 1.5% of Span 80 of the oil phase, 20 g/L of SA, and 1 g/75 g of vanillin-chitosan Schiff base zinc (VCSB-Zn(II)) to SA ratio, and the encapsulation efficiency (EE) was about 65.21%. In comparison to chitosan-Zn(II) (Chs-Zn(II)) and VCSB-Zn(II) released Zn2+ only at simulated gastric fluid, VCSB-Zn(II)@CA microcapsule showed a more continuous release in the whole release process. The cumulative release rate (Ri) of VCSB-Zn(II) and VCSB-Zn(II)@CA was close and much higher than that of Chs-Zn(II), which were 82.81%, 79.15%, and 57.33%, respectively. The release curve met the first-order and the Korsmeyer-Peppas model, which followed the Fick diffusion mechanism. In the future, this Zn2+ sustained released system will lead to a new horizon of more efficient applications in trace element supplements for human beings and animals.