Sensitive electrochemical detection of DR1 based on gold nanoparticle-modified MoS2 and hyaluronic acid-based thionine

Abstract

The analysis of down-regulator of transcription 1 (DR1) offers significant information for the rapid and non-invasive diagnosis of Hashimoto's thyroiditis (HT). In this study, we report a novel dual-signal amplification electrochemical biosensor for the sensitive detection of DR1. Gold nanoparticle (AuNP)-modified molybdenum disulfide (MoS2@AuNPs), which has extremely strong electron transfer ability and abundant binding sites, is first modified on an electrode surface as a substrate material to implement the first signal amplification. After the formation of the sandwich structure based on the specific recognition of antigens and antibodies, the electroactive molecules hyaluronic acid-based thionine (HA@Thi) are introduced to achieve the second signal amplification. Using this dual-signal amplification strategy, the proposed biosensor achieves a linear range of 1 × 10−4–1 × 102 ng mL−1 with a low detection limit of 10.99 fg mL−1. In addition, the electrochemical biosensor has high selectivity and good stability, and is applicable to the assay of DR1 in the presence of complex biological matrices, which is expected to provide a scientific approach for the clinical application of serum DR1 monitoring. More importantly, our method may extend the application of protein-based biosensors in disease diagnosis techniques.

Graphical abstract: Sensitive electrochemical detection of DR1 based on gold nanoparticle-modified MoS2 and hyaluronic acid-based thionine

Article information

Article type
Paper
Submitted
16 Aug 2024
Accepted
01 Oct 2024
First published
09 Apr 2025
This article is Open Access
Creative Commons BY-NC license

Sens. Diagn., 2025, Advance Article

Sensitive electrochemical detection of DR1 based on gold nanoparticle-modified MoS2 and hyaluronic acid-based thionine

Y. Zhang, G. Si, Z. Wang, Y. Wang, X. Cui, H. Yang, F. Si and Y. Liu, Sens. Diagn., 2025, Advance Article , DOI: 10.1039/D4SD00286E

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