Effect of seawater acidification on energy metabolism in the hydrocoral Millepora alcicornis: inhibition of citrate synthase activity indicates disruption in aerobic pathways

Abstract

Ocean acidification is a major threat to coral reefs worldwide, with reduced growth already reported in the hydrocoral Millepora alcicornis (Linnaeus, 1758) under these conditions. Inhibition of enzymes related to energy metabolism is hypothesized as one of the mechanisms associated with the physiological impacts of ocean acidification. Therefore, a mesocosm experiment was conducted to investigate whether three levels of decreasing seawater pH could alter the activity of key enzymes involved in the energy metabolism in M. alcicornis. Hydrocorals were acclimated to marine mesocosm conditions for 20 days and then exposed to different seawater pH levels [ambient pH (8.1) and experimental pH (7.8, 7.5 and 7.2)] for 16 and 30 days. Endpoints analyzed included the activity of key enzymes involved in the regulation of the glycolytic pathway (hexokinase and pyruvate kinase), aerobic energy production via the Krebs cycle (citrate synthase) and anaerobic energy production via lactate formation (lactate dehydrogenase). The results obtained show that only citrate synthase was affected by seawater acidification, as a marked reduction in its activity was observed at all experimental pH levels tested (7.8, 7.5 and 7.2). This finding indicates that reduced growth previously reported for M. alcicornis under seawater acidification conditions can be explained, at least in part, by a negative impact on the Krebs cycle, a major pathway involved in aerobic energy production.