Issue 38, 2011
From the journal:

Chemical Communications

Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase†‡

Jamie R. Rich,a   Anna-Maria Cunningham,b   Michel Gilbertb  and  Stephen G. Withers*a  

* Corresponding authors

a Department of Chemistry and Centre for High-Throughput Biology, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia, Canada
E-mail: withers@chem.ubc.ca
Fax: 604 822 8869
Tel: 604 822 3402

b Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada

Abstract

Glycosynthase mutants of Rhodococcus sp. endo-glycoceramidase II efficiently synthesize complex glycosphingolipids. Glycosyl fluoride donors may be assembled via sequential glycosyltransferase-catalysed glycosylation of lactosyl fluoride. Alternatively, lactosyl fluoride may be coupled to sphingosine prior to subsequent glycosylation steps.

Graphical abstract: Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase

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Article information

DOI
https://doi.org/10.1039/C1CC13885E
Article type
Communication
Submitted
30 Jun 2011
Accepted
16 Aug 2011
First published
30 Aug 2011

Chem. Commun., 2011,47, 10806-10808

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Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase

J. R. Rich, A. Cunningham, M. Gilbert and S. G. Withers, Chem. Commun., 2011, 47, 10806 DOI: 10.1039/C1CC13885E

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