Issue 20, 2012
From the journal:

Lab on a Chip

A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils†‡§

Sewoon Han,a   Ji-Jing Yan,b   Yoojin Shin,a   Jessie J. Jeon,c   Jihee Won,a   Hyo Eun Jeong,a   Roger D. Kamm,cd   Young-Joon Kim*b  and  Seok Chung*a  

* Corresponding authors

a School of Mechanical Engineering, Korea University, South Korea
E-mail: sidchung@korea.ac.kr
Fax: +82-2-3290-3352
Tel: +82-2-2123-2628

b Department of Integrated Omics for Biomedical Science, WCU Program of Graduate School, Yonsei University, South Korea
E-mail: yjkim@yonsei.ac.kr
Fax: +82-2-312-8834
Tel: +82-2-2123-2628

c Department of Mechanical Engineering, Massachusetts Institute of Technology, USA

d Department of Biological Engineering, Massachusetts Institute of Technology, USA

Abstract

Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.

Graphical abstract: A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2LC40445A
Article type
Communication
Submitted
30 Apr 2012
Accepted
03 Aug 2012
First published
06 Aug 2012

Lab Chip, 2012,12, 3861-3865

Permissions

Request permissions

A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils

S. Han, J. Yan, Y. Shin, J. J. Jeon, J. Won, H. Eun Jeong, R. D. Kamm, Y. Kim and S. Chung, Lab Chip, 2012, 12, 3861 DOI: 10.1039/C2LC40445A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements