Issue 6, 2012
From the journal:

RSC Advances

A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules†

Timo Koopmans,a   Frank J. Dekkerb  and  Nathaniel I. Martin*a  

* Corresponding authors

a Department of Medicinal Chemistry & Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, David de Wied Building, Office: 5.64, Universiteitsweg 99, 3584 CG Utrecht, The Netherlands
E-mail: n.i.martin@uu.nl
Tel: +31 (0)6 1878 5274

b Department of Pharmaceutical Gene Modulation, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Groningen, 9713 AV, The Netherlands

Abstract

A new photocleavable affinity tag for use in the enrichment of alkyne-labelled biomolecules is reported. The tag is prepared via a concise synthetic route using readily available materials. The photolytic conditions employed for cleavage of the tag provide for a clean release of enriched biomolecules from streptavidin resin.

Graphical abstract: A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules

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Article information

DOI
https://doi.org/10.1039/C2RA20082A
Article type
Communication
Submitted
13 Jan 2012
Accepted
17 Jan 2012
First published
18 Jan 2012

RSC Adv., 2012,2, 2244-2246

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A photocleavable affinity tag for the enrichment of alkyne-modified biomolecules

T. Koopmans, F. J. Dekker and N. I. Martin, RSC Adv., 2012, 2, 2244 DOI: 10.1039/C2RA20082A

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