Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification

Tian Tian; Heng Xiao; Xiaolian Zhang; Shuang Peng; Xiaoe Zhang; Shan Guo; Shaoru Wang; Songmei Liu; Xin Zhou; Craig Meyers; Xiang Zhou

doi:10.1039/C2CC36728A

Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification†

Tian Tian,‡^a Heng Xiao,‡^a Xiaolian Zhang,^b Shuang Peng,^a Xiaoe Zhang,^a Shan Guo,^a Shaoru Wang,*^a Songmei Liu,^c Xin Zhou,^c Craig Meyers^d and Xiang Zhou*^ae

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* Corresponding authors

^a College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan, P. R. China
E-mail: xzhou@whu.edu.cn, srwang@whu.edu.cn
Fax: +86-27-68756663

^b State Key Laboratory of Virology, Department of Immunology, School of Medicine, Wuhan University, P. R. China

^c Zhongnan Hosptial, Wuhan University, P. R. China

^d Department of Microbiology and Immunology H107, The Penn State College of Medicine, Hershey, U. S. A.

^e State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, P. R. China

Abstract

We reported an efficient strategy based on a strand displacement amplification for serum miRNA detection. In such a system, a multiplexed, sensitive and quick detection of miRNAs could be achieved through a combination of fluorescence labeled probes, a common primer and a polymerase. This could be potentially used in the clinical field to achieve early disease diagnosis and prognosis.

Chemical Communications

Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification†

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Simultaneously sensitive detection of multiple miRNAs based on a strand displacement amplification

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