DNA directed chemistry is commonly performed by using nanomolar amounts of DNA templates. Herein we introduce a method that allows the use of attomolar template loads. A DNA templated native chemical ligation yields to the covalent fixation of two fluorophores while the template is being produced during polymerase
A. Roloff and O. Seitz, Chem. Sci., 2013, 4, 432 DOI: 10.1039/C2SC20961F
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