Issue 1, 2015
From the journal:

Chemical Communications

Label-free, isothermal and ultrasensitive electrochemical detection of DNA and DNA 3′-phosphatase using a cascade enzymatic cleavage strategy†

Shufeng Liu,*a   Tao Liua  and  Li Wanga  

* Corresponding authors

a Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China
E-mail: sliu@qust.edu.cn

Abstract

Label-free and ultrasensitive electrochemical assays of target DNA and T4 polynucleotide kinase phosphatase (PNKP) were developed, which took full advantage of three enzymes to realize the signal readout and amplification. It can ultimately achieve the low detection limits of 10 fM and 1 mU mL−1 for target DNA and PNKP, respectively.

Graphical abstract: Label-free, isothermal and ultrasensitive electrochemical detection of DNA and DNA 3′-phosphatase using a cascade enzymatic cleavage strategy

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Article information

DOI
https://doi.org/10.1039/C4CC08140D
Article type
Communication
Submitted
14 Oct 2014
Accepted
31 Oct 2014
First published
31 Oct 2014

Chem. Commun., 2015,51, 176-179

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Label-free, isothermal and ultrasensitive electrochemical detection of DNA and DNA 3′-phosphatase using a cascade enzymatic cleavage strategy

S. Liu, T. Liu and L. Wang, Chem. Commun., 2015, 51, 176 DOI: 10.1039/C4CC08140D

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