Synchronous sensing of three conserved sequences of Zika virus using a DNAs@MOF hybrid: experimental and molecular simulation studies

Abstract

A metal–organic framework of Cu(II) supported by a zwitterionic pyridinium dicarboxylate and 4,4′-bipyridine mixed ligands was prepared and impregnated with three dye-labeled DNA sequences with fixed wavelength differences. This hybrid material formed is capable of the synchronous detection of three conserved Zika virus RNA sequences with high sensitivity and selectivity, and low detection limits of 0.56 ± 0.02, 0.16 ± 0.04, and 0.19 ± 0.05 nM, without any cross-reactions, as confirmed by both experimental and molecular simulation studies.