Issue 1, 2022
From the journal:

Analyst

Combination of ultrashort PCR and Pyrococcus furiosus Argonaute for DNA detection†

Ruyi He, ORCID logo ab   Longyu Wang,b   Fei Wang,b   Jun Yang,b   Xiao Yu,c   Yuan Wang,d   Zhiguo Liu,*a   Chunhua Li*b  and  Lixin Ma*b  

* Corresponding authors

a School of Life Science and Technology, Wuhan Polytechnic University, China
E-mail: zhiguo_l@126.com

b State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei, People's Republic of China
E-mail: xgspring72@hubu.edu.cn, malixing@hubu.edu.cn

c Hubei Provincial Center for Disease Control and Prevention, Wuhan, Hubei, People's Republic of China

d Medical College of Hubei University of Arts and Sciences, Xiangyang, Hubei, People's Republic of China

Abstract

A simple and user-friendly nucleic acid sensing platform with 10 aM sensitivity, named USPCRP (combines ultrashort PCR with Pyrococcus furiosus Argonaute cleavage for nuleic acids detection) is reported. The product of this ultrashort PCR could be directly used as a DNA guide to mediate PfAgo cleavage of molecular beacons.

Graphical abstract: Combination of ultrashort PCR and Pyrococcus furiosus Argonaute for DNA detection

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Article information

DOI
https://doi.org/10.1039/D1AN01521D
Article type
Communication
Submitted
22 Aug 2021
Accepted
10 Nov 2021
First published
30 Nov 2021

Analyst, 2022,147, 35-39

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Combination of ultrashort PCR and Pyrococcus furiosus Argonaute for DNA detection

R. He, L. Wang, F. Wang, J. Yang, X. Yu, Y. Wang, Z. Liu, C. Li and L. Ma, Analyst, 2022, 147, 35 DOI: 10.1039/D1AN01521D

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