Turn-on fluorescent probe based on dicyanoisophorone for bioimaging and rapid detection of peroxynitrite in aqueous media

Abstract

A novel dicyanoisophorone-based colorimetric fluorescent probe 3 has been prepared for recognizing peroxynitrite in aqueous conditions. A large bathochromic shift of the absorption band is observed upon titration with ONOO, inducing a clearly visible solution color change from yellow to pale pink, which makes “naked-eye” detection possible. Moreover, probe 3 can react instantly with ONOO and is accompanied by a significant fluorescence enhancement at 621 nm while the detection limit is as low as 37 nM. Most importantly, probe 3 exhibits high selectivity and sensitivity towards ONOO in the presence of other competitive ions in aqueous solution. Probe 3 has also been successfully applied in living MCF-7 cells, and the results suggest that probe 3 could be applied as a potential candidate for the detection of ONOO.

Graphical abstract: Turn-on fluorescent probe based on dicyanoisophorone for bioimaging and rapid detection of peroxynitrite in aqueous media

Supplementary files

Article information

Article type
Paper
Submitted
19 Sep 2024
Accepted
27 Oct 2024
First published
28 Oct 2024

Anal. Methods, 2024, Advance Article

Turn-on fluorescent probe based on dicyanoisophorone for bioimaging and rapid detection of peroxynitrite in aqueous media

J. Wu, J. Li, Y. Shi, L. Jiang, C. Chan, R. Feng, Y. Wang and Z. Xue, Anal. Methods, 2024, Advance Article , DOI: 10.1039/D4AY01721H

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