Issue 55, 2024

Mechanistic characterisation of the diterpene synthase for clitopilene and identification of isopentalenene synthase from the fungus Clitopilus passeckerianus

Abstract

Two terpene synthases from the pleuromutilin producing fungus Clitopilus passeckerianus were functionally characterised. The first enzyme CpTS1 produces the new diterpene clitopilene with a novel 6-6-5-5 tetracyclic skeleton, while the second enzyme CpTS2 makes the new sesquiterpene isopentalenene. The CpTS1 reaction mechanism was studied in depth using experimental and theoretical approaches.

Graphical abstract: Mechanistic characterisation of the diterpene synthase for clitopilene and identification of isopentalenene synthase from the fungus Clitopilus passeckerianus

Supplementary files

Article information

Article type
Communication
Submitted
10 May 2024
Accepted
17 Jun 2024
First published
18 Jun 2024

Chem. Commun., 2024,60, 7041-7044

Mechanistic characterisation of the diterpene synthase for clitopilene and identification of isopentalenene synthase from the fungus Clitopilus passeckerianus

H. Li, B. Goldfuss and J. S. Dickschat, Chem. Commun., 2024, 60, 7041 DOI: 10.1039/D4CC02286F

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