This study presents a highly sensitive fluorescence biosensor integrating TDT enzyme and CRISPR-Cas12a for precise detection of sperm DNA damage, achieving a detection limit of 0.99 pM and a linear range of 0.001–0.2 nM. Its exceptional sensitivity, simplicity, and versatility make it a transformative tool for reproductive medicine and clinical diagnostics.
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J. Wang, F. Zhang, Z. Liu, Y. Zhou, L. Pei and B. Yan, Analyst, 2025, Accepted Manuscript , DOI: 10.1039/D5AN00031A
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