From the journal:

Chemical Communications

A Thermus thermophilus Argonaute (TtAgo) cleavage-aided isothermal amplification strategy (TAC-IAS) for precise detection of locus-specific DNA methylation†

Fangfang Sun,a   Bingjie Han, ORCID logo b   Yi Zhao,b   Xing Chen,b   Hui Tian,*c   Chenghui Liu ORCID logo d  and  Yuanyuan Sun ORCID logo *b  

* Corresponding authors

a Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China

b Department of Translational Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, Henan, China
E-mail: sunyy@zzu.edu.cn

c School of Cyber Science and Engineering, Zhengzhou University, Zhengzhou 450052, Henan, China
E-mail: tianhui@zzu.edu.cn

d School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062, Shaanxi, China

Abstract

A versatile TtAgo cleavage-aided isothermal amplification strategy (TAC-IAS) is devised for precise detection of DNA methylation with high flexibility and universality compared to methylation-restriction endonuclease, reliably identifying methylation with an abundance of 0.1% in a large pool of unmethylated fragments.

Graphical abstract: A Thermus thermophilus Argonaute (TtAgo) cleavage-aided isothermal amplification strategy (TAC-IAS) for precise detection of locus-specific DNA methylation

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Article information

DOI
https://doi.org/10.1039/D5CC02012C
Article type
Communication
Submitted
10 Apr 2025
Accepted
13 May 2025
First published
16 May 2025

Chem. Commun., 2025, Advance Article

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A Thermus thermophilus Argonaute (TtAgo) cleavage-aided isothermal amplification strategy (TAC-IAS) for precise detection of locus-specific DNA methylation

F. Sun, B. Han, Y. Zhao, X. Chen, H. Tian, C. Liu and Y. Sun, Chem. Commun., 2025, Advance Article , DOI: 10.1039/D5CC02012C

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