Positive effect of a novel protein from Pleurotus eryngii on improving colitis: exploring potential mechanisms from the perspective of intestinal metabolism

Abstract

Pleurotus eryngii (P. eryngii) protein has demonstrated potential anti-inflammatory effects, but the specific mechanism has not been reported. Our research found that P. eryngii protein improved colitis in mice through various pathways, including reducing levels of inflammatory cytokines, promoting oxidative stress homeostasis, and enhancing immune response capacity. 16S rDNA sequencing showed that under the intervention of P. eryngii protein, the relative abundance of the microbiota (Escherichia, Shigella, and Lachnoclostridium) that induced colitis decreased. Metabolomics results showed that the metabolism of lipids and lipid-like molecules was active, with a decrease in the content of steroids (delta 8,14-sterol and DG (14 : 1 (9Z)/16 : 1 (9Z)/0 : 0)) and an increase in the content of terpenoids ((Z)-alpha-bergamotenoic acid, sterebin-D, and alpha-terpineol). Pathway analysis found that the P. eryngii protein activated the cholesterol metabolism pathway through the production of terpenoids, thus promoting the production of very low-density lipoprotein (VLDL) and bile acids, which regulated the level of IL-10 and intestinal homeostasis. Multi-omics joint analysis found that the production of steroid metabolites could be inhibited by Flavonifractor and Sphingomonas, while the growth of harmful bacteria Enterococcus and Lachnoclostridium was likely to be restrained by the terpenoid metabolites. Overall, this study has illustrated that the P. eryngii protein prevented the production of harmful bacteria and activated the cholesterol metabolism pathway to resist inflammatory reactions by regulating the metabolism of lipids and lipid-like molecules.