Transcriptome and proteome analyses reveal the virulence of the Vibrio alginolyticus effector gene vopR

Abstract

Vibrio alginolyticus is an opportunistic infectious pathogen, and its pathogenicity is related to various virulence factors, with the type III secretion system (T3SS) being one of the important systems for secreting virulence factors. vopR is one of the effector genes of V. alginolyticus T3SS1. To investigate its pathogenicity, this study constructed an overexpression vector to express vopR in host cells and analyzed its impact on the mRNA and protein expression levels of host cells. Transcriptomic analysis revealed that overexpression of vopR led to the upregulation of 410 genes and the downregulation of 207 genes. Proteomic analysis showed that 126 proteins were upregulated, and 518 proteins were downregulated. GO enrichment analysis indicated that the differential genes were significantly enriched in various biological processes such as cellular processes, metabolic processes, and biological regulation, as well as in cellular components like cell parts and membranes, and molecular functions such as binding and catalytic activity. KEGG enrichment analysis demonstrated that the differential genes were mainly enriched in metabolic pathways, cancer-related pathways, and the MAPK signaling pathway. The combined analysis of the transcriptome and proteome screened out 144 overlapping differentially expressed genes, with 60 being upregulated and 54 being downregulated. These results suggest that vopR has a significant impact on the cytoskeleton, metabolism, and immune regulation of host cells during the pathogenic process of V. alginolyticus. This study provides a theoretical basis for a deeper understanding of the pathogenic mechanism of V. alginolyticus.

Supplementary files

Transparent peer review

To support increased transparency, we offer authors the option to publish the peer review history alongside their article.

View this article’s peer review history

Article information

Article type
Research Article
Submitted
20 Feb 2025
Accepted
24 Apr 2025
First published
25 Apr 2025

Mol. Omics, 2025, Accepted Manuscript

Transcriptome and proteome analyses reveal the virulence of the Vibrio alginolyticus effector gene vopR

F. Da, S. Cai, L. Xv, M. Tao, S. Chen and B. Li, Mol. Omics, 2025, Accepted Manuscript , DOI: 10.1039/D5MO00010F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements