G4-SLSELEX-Seq-driven discovery of a G4-specific targeting L-RNA aptamer with unique structural features

Abstract

We integrated sequence-guided library design into G4-SLSELEX-Seq, expanding its application to aptamer selection against DNA G-quadruplexes (dG4s). This strategy identified L-Apt.G3, the first L-RNA aptamer exhibiting low nanomolar binding affinity and dual-specificity binding to parallel and antiparallel G4s through a unique binding motif. Additionally, L-Apt.G3 can competitively disrupt dG4–protein interactions.